Essential role of thiol groups in aldehyde dehydrogenases
نویسندگان
چکیده
منابع مشابه
Mechanism of porphobilinogen synthase. Possible role of essential thiol groups.
The inactivation of beef liver porphobilinogen synthase by the alkylating agents iodoacetate and iodoacetamide has been investigated. The synthase can be inactivated by these reagents only if the enzyme has been activated initially by sulfhydryl reducing agents. Inactivation by both iodoacetate and iodoacetamide was the result of modification of cysteinyl residues. A plot of the incorporation o...
متن کاملRole of the essential thiol groups of yeast alcohol dehydrogenase.
1. Yeast alcohol dehydrogenase inactivated by reaction with iodoacetamide retains 85% of the original NADH-binding capacity as measured under conditions of saturating coenzyme concentration. 2. The dissociation constant of the enzyme-NADH complex is unaffected by inactivation of the enzyme with iodoacetamide, and the affinity of the enzyme for NAD(+) and pyridine-3-aldehyde-adenine dinucleotide...
متن کاملAlcohol dehydrogenases and aldehyde dehydrogenases.
T h e structure, function and evolution of alcohol dehydrogenases and aldehyde dehydrogenases have been studied for several years. Recent progress o n these aspects has been considerable and allows extensive conclusions to be drawn about the relationships and properties of these enzymes. Further aspects have also become available for study, such as the regulation of enzyme expression and the an...
متن کاملFunctional specialization of maize mitochondrial aldehyde dehydrogenases.
The maize (Zea mays) rf2a and rf2b genes both encode homotetrameric aldehyde dehydrogenases (ALDHs). The RF2A protein was shown previously to accumulate in the mitochondria. In vitro import experiments and ALDH assays on mitochondrial extracts from rf2a mutant plants established that the RF2B protein also accumulates in the mitochondria. RNA gel-blot analyses and immunohistolocation experiments...
متن کاملAldehyde oxidation. II. Evidence for closely juxtaposed sulfhydryl groups on dehydrogenases.
The reactivity of mercaptans with carbonyl groups has often suggested the involvement of such combinations in the enzymatic transformation of compounds containing the carbonyl function. Although the details of the interaction between protein and substrate remain a matter of controversy, there can be no denial of the intimate involvement of protein sulfhydry1 groups with substrate in at least se...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1957
ISSN: 0306-3283
DOI: 10.1042/bj0670406